Identification Of Steroids In Water By Ion Trap LC/MS/MS

Richard W. Smith, Agilent Technologies
2660 Matheson Blvd E., Mississauga, ON, L4W 5M2

Abstract

The analytical boundaries for the rapid and sensitive identification of eight representative steroids were investigated using different atmospheric pressure ionization sources combined with ion trap LC/MS. The ability of the ion trap mass spectrometer to generate both MS and MS/MS data in a chromatographic time frame (data dependent acquisition) will be demonstrated and how this capability enables the analyst to have greater confidence in the identification of unknown species.